Infectious Diseases (IDs)
Infectious diseases (IDs) are disorders caused by various microorganisms (such as bacteria, viruses, fungi or parasites) and can spread in different ways: directly or indirectly, from one person to another. The human immune system can neutralize harmful effects of pathogens, however, in some cases, infectious diseases can lead to serious complications. In response to the needs of the evolving diagnostics market, GeneMe has developed rapid qPCR tests for accurate in vitro identifications of pathogens causing a number of common diseases.
The tests amplify and detect a specific nucleic acid chain sequence of infectious pathogen in samples using the qPCR or RT-qPCR method. The assay includes forward and reverse primers to amplify a specific DNA sequence, and a dye-labelled probe to recognize a unique site in the amplified sequence.
During the real-time PCR reaction, the DNA polymerase cleaves the specific dye-labelled probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA sequence. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored in real-time by a PCR detection system. Detecting the infectious pathogen is based on detecting the signal from the dye.