Sexually Transmitted Infections (STIs)
About STIs
Sexually transmitted infections (STIs), also known as sexually transmitted diseases (STDs), involve the transmission of a pathogen between sexual partners through different routes of sexual contact; either oral, anal, or vaginal. STIs become a concern and burden on healthcare systems, as many infections go untreated and lead to potentially serious complications.
In an effort to expedite the diagnosis and treatment of sexually transmitted infections, GeneMe has developed qPCR Detection Kits that detect the individual pathogens causing STIs.
Detection Method
The STI tests amplify and detect a specific DNA sequence of an infectious pathogen in purified genomic samples. The STI assay includes forward and reverse primers to amplify a specific DNA sequence, and a FAM dye-labelled probe to recognize a unique site in the amplified DNA sequence.
During the real-time PCR reaction, the DNA polymerase cleaves the specific FAM dye-labelled probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA sequence. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by a PCR detection system. Detecting the infectious pathogen is based on detecting a signal from the FAM-dye.